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internal reflection fluorescence tirf microscopy  (Nikon)


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    Structured Review

    Nikon internal reflection fluorescence tirf microscopy
    (a) Schematic representation of light-controlled inhibition of microtubule polymerisation by proPST-1-loaded polymer nanoparticles (proPST-1@PN). Under dark conditions, the encapsulated photocaged prodrug remains inactive, allowing normal microtubule formation. Upon 365 nm irradiation, photolytic uncaging and release of active cis -PST-1 suppress microtubule assembly. Created in BioRender. Singh, H. (2026) https://BioRender.com/fac5r0eT . (b) Tubulin polymerisation kinetics monitored by absorbance at 340 nm for control samples, empty polymer nanoparticles (PN control), and proPST-1@PN under dark and irradiated conditions. Irradiation of proPST-1@PN results in pronounced inhibition of tubulin polymerisation, whereas no inhibition is observed in the dark. (c) <t>Fluorescence</t> <t>microscopy</t> images of microtubule structures formed in the presence of control samples, proPST-1@PN under dark conditions, and irradiated proPST-1@PN. Normal microtubule growth and network formation are observed in control and dark conditions, while irradiation results in substantial disruption of microtubule assembly. Scale bars: 10 µm.
    Internal Reflection Fluorescence Tirf Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+microscopy+system/N-STORM/pmc13673699-59-1-9
    Average 99 stars, based on 1 article reviews
    internal reflection fluorescence tirf microscopy - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Simultaneous activation and release of a microtubule inhibitor from amphiphilic polymeric nanoparticles"

    Article Title: Simultaneous activation and release of a microtubule inhibitor from amphiphilic polymeric nanoparticles

    Journal: Nanoscale Advances

    doi: 10.1039/d6na00763e

    (a) Schematic representation of light-controlled inhibition of microtubule polymerisation by proPST-1-loaded polymer nanoparticles (proPST-1@PN). Under dark conditions, the encapsulated photocaged prodrug remains inactive, allowing normal microtubule formation. Upon 365 nm irradiation, photolytic uncaging and release of active cis -PST-1 suppress microtubule assembly. Created in BioRender. Singh, H. (2026) https://BioRender.com/fac5r0eT . (b) Tubulin polymerisation kinetics monitored by absorbance at 340 nm for control samples, empty polymer nanoparticles (PN control), and proPST-1@PN under dark and irradiated conditions. Irradiation of proPST-1@PN results in pronounced inhibition of tubulin polymerisation, whereas no inhibition is observed in the dark. (c) Fluorescence microscopy images of microtubule structures formed in the presence of control samples, proPST-1@PN under dark conditions, and irradiated proPST-1@PN. Normal microtubule growth and network formation are observed in control and dark conditions, while irradiation results in substantial disruption of microtubule assembly. Scale bars: 10 µm.
    Figure Legend Snippet: (a) Schematic representation of light-controlled inhibition of microtubule polymerisation by proPST-1-loaded polymer nanoparticles (proPST-1@PN). Under dark conditions, the encapsulated photocaged prodrug remains inactive, allowing normal microtubule formation. Upon 365 nm irradiation, photolytic uncaging and release of active cis -PST-1 suppress microtubule assembly. Created in BioRender. Singh, H. (2026) https://BioRender.com/fac5r0eT . (b) Tubulin polymerisation kinetics monitored by absorbance at 340 nm for control samples, empty polymer nanoparticles (PN control), and proPST-1@PN under dark and irradiated conditions. Irradiation of proPST-1@PN results in pronounced inhibition of tubulin polymerisation, whereas no inhibition is observed in the dark. (c) Fluorescence microscopy images of microtubule structures formed in the presence of control samples, proPST-1@PN under dark conditions, and irradiated proPST-1@PN. Normal microtubule growth and network formation are observed in control and dark conditions, while irradiation results in substantial disruption of microtubule assembly. Scale bars: 10 µm.

    Techniques Used: Inhibition, Polymer, Irradiation, Control, Fluorescence, Microscopy, Disruption

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    Image Search Results


    (a) Schematic representation of light-controlled inhibition of microtubule polymerisation by proPST-1-loaded polymer nanoparticles (proPST-1@PN). Under dark conditions, the encapsulated photocaged prodrug remains inactive, allowing normal microtubule formation. Upon 365 nm irradiation, photolytic uncaging and release of active cis -PST-1 suppress microtubule assembly. Created in BioRender. Singh, H. (2026) https://BioRender.com/fac5r0eT . (b) Tubulin polymerisation kinetics monitored by absorbance at 340 nm for control samples, empty polymer nanoparticles (PN control), and proPST-1@PN under dark and irradiated conditions. Irradiation of proPST-1@PN results in pronounced inhibition of tubulin polymerisation, whereas no inhibition is observed in the dark. (c) Fluorescence microscopy images of microtubule structures formed in the presence of control samples, proPST-1@PN under dark conditions, and irradiated proPST-1@PN. Normal microtubule growth and network formation are observed in control and dark conditions, while irradiation results in substantial disruption of microtubule assembly. Scale bars: 10 µm.

    Journal: Nanoscale Advances

    Article Title: Simultaneous activation and release of a microtubule inhibitor from amphiphilic polymeric nanoparticles

    doi: 10.1039/d6na00763e

    Figure Lengend Snippet: (a) Schematic representation of light-controlled inhibition of microtubule polymerisation by proPST-1-loaded polymer nanoparticles (proPST-1@PN). Under dark conditions, the encapsulated photocaged prodrug remains inactive, allowing normal microtubule formation. Upon 365 nm irradiation, photolytic uncaging and release of active cis -PST-1 suppress microtubule assembly. Created in BioRender. Singh, H. (2026) https://BioRender.com/fac5r0eT . (b) Tubulin polymerisation kinetics monitored by absorbance at 340 nm for control samples, empty polymer nanoparticles (PN control), and proPST-1@PN under dark and irradiated conditions. Irradiation of proPST-1@PN results in pronounced inhibition of tubulin polymerisation, whereas no inhibition is observed in the dark. (c) Fluorescence microscopy images of microtubule structures formed in the presence of control samples, proPST-1@PN under dark conditions, and irradiated proPST-1@PN. Normal microtubule growth and network formation are observed in control and dark conditions, while irradiation results in substantial disruption of microtubule assembly. Scale bars: 10 µm.

    Article Snippet: Total Internal Reflection Fluorescence (TIRF) microscopy was performed on Nikon N-STORM microscopy system via excitation of the sample in green channel (561 nm laser, 10% intensity).

    Techniques: Inhibition, Polymer, Irradiation, Control, Fluorescence, Microscopy, Disruption